Glycoengeneering and Glycotechnology


On 21th June morning, ten reporters made wonderful presentations at the Sea Joy Hall of the Seaview Garden Hotel. Prof. Lianchun Wang and Prof. Peng George Wang chaired the session of "Glycoengeneering and Glycotechnology". The overview of this session is as follows:

Prof. Anne Dell

Professor Anne Dell (Imperial College London, UK) gave the first presentation. In this talk she illustrated how high sensitivity glycomics and glycoproteomics can provide insights into some of these biological processes. Ovarian cancer cell glycomics gave insights into strategies for identifying biologically important low abundance epitopes andgalectin ligands: new insights into the regulation of their expression in malignant melanomas and pregnant

Prof. Ying Zhang

Professor Ying Zhang (Fudan University, China) introduced the MS-based N-glycoproteome and N-glycome analysis. They co-workers explored the methods of enriching the glycopeptidesusing a newly synthesized aminooxy-functionalized magnetic nanoparticle. They also developed a novel N-glycan quantitation approach using metallic element chelated tag labeling (MeCTL) through reductive amination. Their works enable the highly sensitive and specific MS analysis of the glycoproteome and the glycome.

Prof. Fuming Zhang

Professor Fuming Zhang (Rensselaer Polytechnic Institute, USA)’s presentation introduced SPR studies on the interactions between GAGs/heparin and proteins, and the application of SPR in glycomics/glycoengeneering study. He pointed out that SPR is a real time and label-free analytical technology. It is powerful for study on the molecular interaction of GAG and proteins. SPR can provide binding affinity genetics and structural information of the binding molecules.

Prof. Zhenqing Zhang

Professor Zhenqing Zhang (Soochow University, China)’s talk was themed around the importance of glycan sequencing methods development. His lab developed a couple of innovative methods with LC-MS to analyze the sequences of various oligosaccharides. One method was developed with HPAEC linked to a PAD and a Q/Tof-MSin parallel. Another method was developed with SEC linked to mass spectrometry (MS).

After coffee break, Prof. Anne Dell and Prof. Fuming Zhang continued to chair the section of “Glycoengeneering and Glycotechnology”.

Prof. Peng George Wang

Professor Peng George Wang (Georgia State University, USA) discussed three areas of applications of glycosyltransferases for chemistry and biomedicine. The most useful areas are in the bio-synthesis of glycans. Then, he showed a full automation of glycosyltransferase-based synthesis carried out with a commercial peptide synthesizer. Furthermore, glycosyltransferases also can be used to enzymatically label acceptor structures on cells or tissue surfaces.

Prof. Peng Wu

Next, Professor Peng Wu (The Scripps Research Institute, USA) gave a talk about their recent progress towards the development of methods for cell-surface chemoenzymatic glycan engineering. They demonstrated that in situ fucosylation may enhance anti-tumor efficacy of T cells by promoting their homing and viability after adoptive transfer. At the same time, his group constructed some antibody-cell conjugates via chemoenzymatic method. The NK-92-Herceptin conjugates can specifically bind to and kill HER2+ cancer cells.

Prof. Hongzhi Cao

Professor Hongzhi Cao (Shandong University, China) introduced enzymatic modular assembly of human glycans. His group have developed diversity-oriented modular chemoenzymatic strategy for the rapid assembly of various complex glycans in a few steps. Several chemical concepts were successfully employed to themodular system to control the regioselective and vary the target glycan sequence.

Prof. Jürgen Seibel

Professor Jürgen Seibel (University of Wuerzburg, Germany) presented recent developments in bio-organic synthesis, biophysical visualization and bioanalysis based on glycoengineering. They used special synthesized carbohydrate labels in combination with new super-resolution imaging approaches. It allows access to both mapping and identification of cell surface glycoproteins well below the diffraction limit. This methodology will recognize the surface molecules involved in bacterial adherence. Therefore, it has potential implications in viral infection prevention.

Dr. Jine Li

In Doctor Jine Li (University of North Carolina Chapel Hill, USA)’s talk, she showed us an enzymatic method for synthesis of CS oligosaccharides library with high efficiency. The library covers 4-O-sulfated, 6-O-sulfated and 4,6-disulfated chondroitin oligosaccharides ranging up to 19mer-saccharides. And they will test the protective effects in various disease-related animal models.

Prof. Morihisa Fujita

Professor Morihisa Fujita (Jiangnan University, China) showed us HEK293 cell lines with high-mannose-type asparagine (N)-linked, low-complexity glycans. They knocked out three genes encoding Golgi mannosidases (MAN1A1 and MAN1A2) and endoplasmic reticulum mannosidase I (MAN1B1) in HEK293 cells. The triple-KO HEK293 cells are suitable for producing recombinant proteins including lysosomal enzymes with high-mannose-type N-glycans.